cytochalasin d Search Results


cytochalasin d  (MedChemExpress)
97
MedChemExpress cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytochalasin+d/Cytochalasin+D/bio_rxiv__2023__02__04__527154-193-1-14
Average 97 stars, based on 1 article reviews
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97/100 stars
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cytochalasin d  (StressMarq)
90
StressMarq cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by StressMarq, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytochalasin+d/Cytochalasin+D/pmc08790337-79-20-22
Average 90 stars, based on 1 article reviews
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cytochalasin d  (Tocris)
95
Tocris cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytochalasin+d/Cytochalasin+D/pmc04656878-34-0-6
Average 95 stars, based on 1 article reviews
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cytochalasin d sigma aldrich  (Tocris)
94
Tocris cytochalasin d sigma aldrich
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D Sigma Aldrich, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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cytochalasin d  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytochalasin+d/Cytochalasin+D/pmc05669856-120-9-21
Average 94 stars, based on 1 article reviews
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cytochalasin d  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytochalasin d  (Valiant Co Ltd)
93
Valiant Co Ltd cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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cytochalasin d  (Biomol GmbH)
90
Biomol GmbH cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytochalasin+d/cytochalasin+d/pm24440830-125-3-16
Average 90 stars, based on 1 article reviews
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cytochalasin d apexbio  (ApexBio)
90
ApexBio cytochalasin d apexbio
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D Apexbio, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytochalasin d  (Enzo Biochem)
90
Enzo Biochem cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytochalasin+d/cytochalasin+d/pm30557915-55-26-28
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cytochalasin d (cyd  (FUJIFILM)
90
FUJIFILM cytochalasin d (cyd
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D (Cyd, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytochalasin d  (Merck KGaA)
90
Merck KGaA cytochalasin d
a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).
Cytochalasin D, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).

Journal: bioRxiv

Article Title: Vaccinia virus induces EMT-like transformation and RhoA-mediated mesenchymal migration

doi: 10.1101/2023.02.04.527154

Figure Lengend Snippet: a , Representative super-resolution images of a control Vero cell (left) and a VACV-infected Vero cell (right) expressing LifeAct-mRuby. Scale bars represent 10 μm. b , Representative actin stress fiber images (top) and fluorescent quantitative (bottom left) and width (bottom right) of analysis stress fibers in control and VACV-infected cells by phalloidin staining. Scale bar represents 5 μm. c , Representative immunofluorescence images of F-actin (phalloidin, green) and p-MLC (red) in the control and VACV-infected cell. Scale bar represents 20 μm. d , e , Immunoblotting (d) and quantitative (e) analysis of MLC and p-MLC in Vero cells during VACV infection (n=3). f , g , Montage of frames from live-cell imaging (f) and relative fluorescent intensity analysis (g) of control and VACV-infected Vero cells expressing LifeAct-mRuby. The dotted lines represent the cell outlines (blue: control cells; red: VACV-infected cells). Scale bar represents 40 μm. h , Schematic diagram of the process of VICM accompanied by stress fibers depolymerization, leading protrusion formation, and rear contraction. i , j , Time-lapse actin heatmap (i) and plot profile of gray value (j) along white dotted line in a control cell in two hours. Scale bar represents 20 μm. k, l , Time-lapse actin heatmap (k) and plot profile of gray value (l) along white dotted line in a VACV-infected cell in two hours. Scale bar represents 20 μm. m , n , MSD (m) and representative images of actin cytoskeleton distribution (n) of Vero-LifeAct-mRuby cells during VACV infection after 250 nM jasplakinolide, 1 μM cytochalasin D, 10 μM CK666, and 1 μM latrunculin A treatment. Scale bar represents 20 μm. Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test).

Article Snippet: Jasplakinolide, cytochalasin D, latrunculin A, Y15, tetracycline, ML-7 hydrochloride, and ML-9 were purchased from MedChemExpress.

Techniques: Control, Infection, Expressing, Staining, Immunofluorescence, Western Blot, Live Cell Imaging